Detection by PCR of the VNTR polymorphism at D4S95
نویسندگان
چکیده
منابع مشابه
PCR detection of the TAQ1 polymorphism at the CA2 locus.
In humans, at least seven isozymes of carbonic anhydrase (CA1—CA7) are known, five of which are encoded by genes on chromosomes 1 (CA6), 8 (CA1-3) and 16 (CAT) (1). We have previously described a Taql RFLP with a 50% allele frequency near the CA2 gene (2). We have now bracketed the site with PCR (3) primers to develop an STS site. The original DNA samples (2) produced the same typings (+ = Taql...
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PCR detection of a biallelic polymorphism at D4S43.
Source and Description: The DNA marker pKP 1.65, is a single copy probe for the D4S43 locus. The 1.7 kb PstI fragment reveals a VNTR (1). Recently, the detection of the VNTR by PCR, after sequencing the adjacent 1269 bp PstI fragment was described (2). In order to construct primers for PCR we have sequenced the 1652 bp PstI fragment of pKP 1.65. A guanosine rich sequence in the last third of th...
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Detection by PCR of the VNTR polymorphism at D4S95 Bernice A.AIIitto, Glenn T.Horn, Michael R.AItherr, Brenda Richards, Andrea I.McClatchey, John J.Wasmuth and James F.Gusella* Neurogenetics Laboratory, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Boston, MA 02114, department of Human Genetics, Integrated Genetics, One Mountain Road, Framingham, MA 01752 an...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.14.4015-a